ISOLATION OF CF0CF1 FROM CHLAMYDOMONAS-REINHARDTII CW15 AND THE N-TERMINAL AMINO-ACID-SEQUENCES OF THE CF0CF1 SUBUNITS

CF0CF1 was isolated from chloroplasts of the cell wall-deficient Chlam ydomonas reinhardtii strain cw15. The subunit pattern was analyzed by SDS-gel electrophoresis and the IV-terminal amino acid sequences of al l nine subunits were determined by microsequencing. The amino acid seq uences of subunits alpha, beta, gamma and epsilon match with those der ived from the corresponding Chlamydomonus DNA sequences, In variance w ith the previously assumed N-terminus of beta; however it was found th at the first 11 amino acids are lacking, The subunits delta, I, II, II I and IV were identified by comparison with known sequences of homolog ous polypeptides of higher plant chloroplasts and cyanobacteria, respe ctively.