SURVEY ON SWINE SINES (PRE-1) AS CANDIDATES FOR SSCP MARKERS IN GENETIC-LINKAGE ANALYSIS

Authors
HARUMI T KIMURA M YASUE H
Citation
T. Harumi et al., SURVEY ON SWINE SINES (PRE-1) AS CANDIDATES FOR SSCP MARKERS IN GENETIC-LINKAGE ANALYSIS, Animal genetics, 26(6), 1995, pp. 403-406
Citations number
16
Categorie Soggetti
Genetics & Heredity","Veterinary Sciences
Journal title
Animal genetics → ACNP
ISSN journal
02689146
Volume
26
Issue
6
Year of publication
1995
Pages
403 - 406
Database
ISI
SICI code
0268-9146(1995)26:6<403:SOSS(A>2.0.ZU;2-Z
Abstract
Of 310 random cosmid clones, 216 were positive for PRE-1 sequences by Southern hybridization. Thirty nine sub-fragments positive for the PRE -1 sequences were cloned from independent cosmid clones, and sequenced , with 17 complete PRE-1 elements found. Seven PRE-1 loci were amplifi ed by polymerase chain reaction using genomic DNA of 12 unrelated pigs as template. The amplified fragments were then subjected to an analys is of single strand conformation polymorphism, with all the loci being polymorphic.