Using Kimura's distance measure we have calculated the average age of
all major Alu subfamilies based on the most recent available data. We
conclude that AluJ sequences are some 26 Myr older than previously tho
ught. Furthermore, the origin of the FLA (Free Left Arm) Alu family ca
n be traced back to the very beginning of the mammalian radiation. One
new minor subfamily is reported and discussed in the context of seque
nce diversity in major Alu subfamilies.