GENOMIC METHYLATION PATTERNS OF THE DUNNING R-3327 PROSTATE ADENOCARCINOMA SYSTEM

Genomic methylation patterns of Dunning R-3327 cell lines anaplastic t umor-1 (AT-1), anaplastic tumor-3 (AT-3), metastasis-lymph and lung (M at-LyLu) and metastasis-lung (Mat-Lu), and Mat-LyLu cells treated with difluoromethylornithine (DEMO), and retinoic acid (RA) have been anal yzed. Each cell line was digested with HpaII and MspI restriction endo nuclease enzymes to characterize methylation patterns, at the interior cytosine of the sequence C(m)CGG. Identical molecular weight banding patterns were found for both HpaII and MspI digests in normal dorsal p rostate (NDP) used as a control. Both the treated and non-treated Dunn ing R-3327 cells digested with HpaII and MspI, displayed similar bandi ng profiles from those seen in NDP solid tissues, indicative of a prog ressive loss of methylation at CCGG sites.