AUTOREGULATION OF ICLR, THE GENE ENCODING THE REPRESSOR OF THE GLYOXYLATE BYPASS OPERON

The aceBAK operon was partially induced by a multicopy plasmid which c arried the promoter region of the gene which encodes its repressor, ic lR. Gel shift and DNase I analyses demonstrated that IclR binds to its own promoter. Disruption of iclR increased the expression of an iclR: :lacZ operon fusion. Although aceBAK and iclR are both regulated by Ic lR, aceBAK expression responds to the carbon source, while expression of iclR does not.