TURBIDIMETRIC ASSAY OF ANT-EGG GLYCOPROTEIN LECTIN INTERACTION

Ant (Oecophylla smaragdina Fabr.) eggs glycoprotein was found to have interacted with a panel of lectins, viz., jacalin, peanut, wheatgerm, Concanavalin A, and Ficus cunia and the extent of interaction together with thermodynamic parameters of the reactions were investigated turb idimetrically. The association constants (K-a) of ant egg glycoprotein -lectins interactions were determined by ultraviolet absorption differ ence spectroscopy and showed strongest binding between jacalin and ant egg glycoprotein. The reactivity of lectins to ant egg glycoprotein w as dependent on pH and temperature of the reaction medium.