SIMILAR BINDING-PROPERTIES FOR A NEUTRALIZING ANTITETANUS TOXOID HUMAN MONOCLONAL-ANTIBODY AND ITS BACTERIALLY EXPRESSED FAB

Authors
LAFAYE P NATO F MAZIE JC DOYEN N
Citation
P. Lafaye et al., SIMILAR BINDING-PROPERTIES FOR A NEUTRALIZING ANTITETANUS TOXOID HUMAN MONOCLONAL-ANTIBODY AND ITS BACTERIALLY EXPRESSED FAB, Research in immunology, 146(6), 1995, pp. 373-382
Citations number
43
Categorie Soggetti
Immunology
Journal title
Research in immunology → ACNP
ISSN journal
09232494
Volume
146
Issue
6
Year of publication
1995
Pages
373 - 382
Database
ISI
SICI code
0923-2494(1995)146:6<373:SBFANA>2.0.ZU;2-I
Abstract
A high-affinity anti-tenanus toroid (TT) human monoclonal antibody sho wing neutralizing activity was isolated from a fusion between mouse my eloma and human splenic cells. Fab fragments from this antibody were o btained using a recombinant phage surface-display expression system. T he parental antibody and the corresponding Fab had identical immunolog ical activities, including specificity and affinity. These results con firm the feasibility of developing Escherichia coli expression of mono clonal human Fab from hybridoma cells.