HUMAN CD4(-LYMPHOCYTES ARE BOTH CYTOTOXIC TO TOXOPLASMA GONDII-INFECTED CELLS() AND CD8(+) T)

Studies to determine if Toxoplasma gondii-specific human T cells lyse parasite-infected cells have yielded conflicting results. Furthermore, attempts to obtain human cytotoxic CD8(+) T lymphocytes have been dif ficult because of the lack of a reproducible system for their generati on. By using paraformaldehyde-fixed, T. gondii-infected peripheral blo od mononuclear cells as antigen-presenting cells, we developed a metho d whereby T. gondii-specific T-tell lines can be reproducibly generate d, Six T. gondii-specific T-cell lines were generated from an individu al chronically infected with T. gondii. Cytofluorometric analysis of t hese lines revealed > 99% CD3(+), 85 to 95% CD3(+) alpha beta T-cell-r eceptor-positive (TCR(+)), 5 to 9% CD3(+) gamma delta TCR(+), 50 to 70 % CD4(+), and 20 to 40% CD8(+) cells when cells were examined during t he first 3 weeks of stimulation and >99% CD3(+), >99% CD3(+) alpha bet a TCR(+), <1% CD3(+) gamma delta TCR(+), 20 to 40% CD4(+), acid 60 to 80% CD8(+) cells when cells were examined between 5 and 11 weeks, Both CD4(+) and CD8(+) T cells had remarkable cytotoxic activity against T . gondii-infected target cells (30 to 50% specific Cr release at an ef fector-to-target ratio of 30:1) but not against uninfected target cell s (<10% at an effector-to-target ratio of 30:1). Cytotoxic activity by the whole T-cell lines was not T, gondii strain specific, Whole T-cel l lines were cytotoxic for target cells infected with the C56 and ME49 strains and the RH strain (which was used to infect peripheral blood mononuclear cells), T. gondii specific T-cell lines displayed the pred ominant expression of V beta 7 TCR. The CDR3 regions of the V beta 7 T CRs of these T-cell lines showed a striking degree of sequence identit y (oligoclonality), T-cell lines obtained by the method reported here can be used to characterize functional activity of T-lymphocyte subset s in humans infected with T. gondii.