DETERMINATION OF TOTAL, AND BOUND SE IN SERA BY INAA

Authors
SPATEVL,"MASON MM REAMS CL BASKETT CK MORRIS JS MILLS DS
Citation
Mm. Spatevl,"mason et al., DETERMINATION OF TOTAL, AND BOUND SE IN SERA BY INAA, Journal of radioanalytical and nuclear chemistry, 179(2), 1994, pp. 315-322
Citations number
2
Categorie Soggetti
Chemistry Analytical","Nuclear Sciences & Tecnology","Chemistry Inorganic & Nuclear
Journal title
Journal of radioanalytical and nuclear chemistry → ACNP
ISSN journal
02365731
Volume
179
Issue
2
Year of publication
1994
Pages
315 - 322
Database
ISI
SICI code
0236-5731(1994)179:2<315:DOTABS>2.0.ZU;2-P
Abstract
In this study we report on the comParison between the total selenium i n serum (''total Se'') with that which is apparently bound to high mol ecular weight ( > 12,000 D) species, presumably proteins (''bound Se'' ). Nine hundred seventy seven (977) serum samples arising out of a pop ulation-based epidemiological study were prepared in duplicate for the determination of ''total Se'' by pipeting directly into irradiation v ials; and separate duplicate aliquots were dialyzed against DI water f or the determination of ''bound Se''. All samples were analyzed by neu tron activation analysis via Se-77m (17.4 s). A small dialyzable Se co mponent (approximately 6%) (''free Se''), defined as the difference be tween the ''total Se'' minus the ''bound Se'', was identified.