BENEFICIAL EFFECT OF THROMBOXANE A(2) SYNTHETASE INHIBITOR (OKY-046) ON 24-HR SIMPLE HYPOTHERMIC PRESERVATION OF THE CANINE PANCREAS GRAFT

This study was performed to investigate the potential protective effec t of OKY-046, an inhibitor of thromboxane A2 synthetase, on the viabil ity of 24-hr preserved canine segmental pancreas graft, Each graft was preserved in either cold Euro-Collins (E-C) solution (control group;n =5) or E C+OKY-046(10(-4)M) solution (OKY-046 group;n=5), In both grou ps, grafts were preserved for 24 hr and calculated the total levels of 6keto-prostaglandin F-1 alpha (6ket-PGF(1) alpha) and thromboxane B-2 (TxB(2)) in the preservation solution per pancreas graft weight at the time point of 0,3,7,12,24 hr after preservation, The efficacy of OKY- 046 in protecting ischemically stressed grafts was examined by histolo gical findings of the grafts before transplantation and blood flow of the transplanted pancreas graft, During 24-hr preservation time, incre ased production of 6ket-PGF(1) alpha and decreased production of TxB(2 ) were found in OKY-046 group, Histologically ischemic damage of the a cinar cells was observed in the control group, but it was milder in th e OKY-046 group, The blood flow of the transplanted pancreas in the OK Y-046 group were higher than that in control group, In the OKY-046 gro up, all dogs survived with functioning grafts, although some grafts we re lost in the control group, These results suggest that there is a po ssibility that OKY-046 may contribute to the improvement of the surviv al rate in segmental pancreas transplantation.