MAPPING OF 22 NOTI LINKING CLONES ON HUMAN-CHROMOSOME-3 BY POLYMERASECHAIN-REACTION AND SOMATIC-CELL HYBRID PANELS

Twenty-two human chromosome 3 derived and partially sequenced Notl lin king clones were mapped using two somatic cell hybrid panels. Somatic cell hybrid mapping was performed by Southern hybridization and/or by polymerase chain reaction (PCR), using 300-500 bp CpG-rich sequences s urrounding Notl sites. Thus, 22 new Notl site-tagged (sequence tagged sites) STSs were created, distributed over the entire human chromosome 3. The majority of these linking clones tag known or unknown expresse d sequences (genes). Together with other physical and genetic mapping methods, localization of Notl linking clones facilitates the construct ion of a long-range physical map and, at the same time, a transcriptio nal map of human chromosome 3.