Well-defined G-bands were obtained on metaphase chromosomes from Tapin
oma nigerrimum using trypsin and warm 2 x SSC in sequence. The G-bande
d pattern allowed the identification of all chromosomes. Evidence for
asynchronous condensation of the chromosomes of this species is provid
ed. Different banding patterns were obtained when metaphase chromosome
s were stained with DA/DAPI alone and with DA/DAPI after a standard G-
banding procedure. The G-banding phenomenon is discussed using the res
ult obtained.