EVALUATION OF DIFFERENT PARAMETERS AND THEIR INFLUENCE ON THE PEG (POLYETHYLENE-GLYCOL) MEDIATED GENE-TRANSFER INTO RICE (ORYZA-SATIVA L) PROTOPLASTS

This work was concentrated in the analysis of the PEG (polyethylene gl ycol) mediated genetic transformation method for rice protoplast. The optimisation of the transformation efficiency was made by the analysis of the transient expression of the gusA gene in protoplasts, the meth od of analysis chosen was the fluorometric assay. The protoplasts were isolated from cell suspensions of rice. In this work a technical impr ovement was achieved by optimising the transformation rates for rice p rotoplast transformation with the PEG method. Some parameters as gradu al dilution, amount of plasmid DNA, PEG concentration, and protoplast density proved to be very important for the PEG-mediated transformatio n efficiency.