EVALUATION OF CHROMATOGRAPHIC METHODS FOR THE DETERMINATION OF NIFEDIPINE IN HUMAN SERUM

Gas-liquid chromatography and high-performance liquid chromatography w ere compared for the identification and determination of nifedipine in biological samples and the elaboration of the optimum liquid-liquid e xtraction procedure. The determination limits were 2 and 10 ng/ml, res pectively, and the detection limits were 1 and 5 ng/ml, respectively. The calibration graphs were linear in the ranges 2-300 and 10-500 ng/m l, respectively. Recoveries based on three different concentrations we re 88.7-95.8% and 93.7-104.2%, respectively. Both methods are sensitiv e, specific and reproducible enough for pharmacokinetic studies and th erapeutic drug monitoring.