REVERSED-PHASE RETENTION CHARACTERISTICS OF SOME BIOACTIVE HETEROCYCLIC-COMPOUNDS

The lipophilicities and specific hydrophobic surface areas of sixteen bioactive heterocyclic compounds were determined by reversed-phase thi n-layer chromatography using various eluent systems. The data were eva luated by principal component analysis (PCA), separately calculated fr om the correlation and covariance matrices, and by cluster analysis. T he ratio of the variances explained by the first PC component was high and was very similar for both PCA methods, suggesting that the eluent s have common elution characteristics. The lipophilicities and specifi c hydrophobic surface areas of the compounds are well separated on the two-dimensional non-linear maps of PC variables, indicating the diffe rent information contents of the two physico-chemical parameters. The information contents of cluster analysis and two-dimensional non-linea r mapping techniques were found to be similar but not identical.