M. Tada et al., ANALYSIS OF K-RAS GENE MUTATION IN HYPERPLASTIC DUCT CELLS OF THE PANCREAS WITHOUT PANCREATIC DISEASE, Gastroenterology, 110(1), 1996, pp. 227-231
Background & Aims: We and others have previously shown that the mutati
on of K-ras codon 12 was found in the majority of pancreatic adenocarc
inomas. The mutation has also been identified in the pancreatic duct w
ith mucous cell hyperplasia in association with chronic pancreatitis,
Ductal hyperplasia is also frequently found in the pancreas free from
pancreatic carcinoma or chronic pancreatitis. The aim of this study wa
s to assess the incidence and types of mutations in hyperplastic foci
in these cases. Methods:The nucleotide sequence of the K-ras gene at c
odon 12 of the DNA extracted from microdissected hyperplastic epitheli
um of the pancreatic duct obtained at autopsy in patients without panc
reatic adenocarcinoma or chronic pancreatitis was analyzed, Results: O
f 38 patients with 79 hyperplastic foci, 12 patients (with 19 hyperpla
stic foci) had mutations. None of the 16 normal ducts in 12 specimens
had this mutation. The nucleotide sequence of the codon in 53% of duct
al hyperplastic foci was TGT or AGT, both of which were not found in 3
0 cases of adenocarcinoma. Conclusions: These results suggest that the
res gene mutation occurs frequently in multifocal hyperplastic foci o
f pancreatic duct and that the mutations may not have direct relevance
to the carcinogenesis of pancreatic cancer.