DNA POLYMORPHISM DETECTION IN TRIBOLIUM-CASTANEUM (HERBST) (COLEOPTERA, TENEBRIONIDAE) - POTENTIAL USE IN STORED-PRODUCT PEST-MANAGEMENT

Different pairs of oligonucleotide primers have used for the detection of DNA polymorphism between Tribolium castaneum strains. Amplificatio n of a major DNA fragment and two minor was observed in all strains te sted excepted for one strain from France which present only two major bands (respectively at 800 bp and 880 bp). Arbitrarily, the major band amplified from a DNA sample of the strain originating from the Philip pines was choosen as a probe (sequence of 487 bp in length). The combi nation of this probe and restriction enzymes permitted the discriminat ion between strains. Our results show that RAP marker of Tribolium cas taneum could be used to study its population genetics.