PROCESSING LUNG-TISSUE WITHOUT WATER AND ORGANIC-SOLVENTS

Infusion of melted paraffin into the mouse lung makes it possible to c ut and process the dried-out lung tissue without water and organic sol vents. The lung filled with paraffin is attached to the block and cut as conventional paraffin embedded samples that can be examined under t he microscope. Alternatively, lung sections can be infiltrated with pa raffin oil to remove the solidified paraffin from the alveoli and to c larify the tissue. This technique was applied to the detection of quin ine distribution in the lung tissue.