NMR AND CRYSTALLOGRAPHY - COMPLEMENTARY APPROACHES TO STRUCTURE DETERMINATION

A knowledge of the three-dimensional structure of a protein is essenti al to understand how a protein performs its functions. It is also a pr ime requirement for the rational design of novel sequences with specif ic structural, chemical or catalytic properties. Until recently, such information could only be obtained from X-ray diffraction studies on p rotein crystals. In the past few years, however, nuclear magnetic reso nance (NMR) spectroscopy in solution has rapidly become established as an effective alternative method. This review briefly examines the two techniques and their relevance to protein engineering and design.