MOLECULAR-CLONING AND EXPRESSION OF ALPHA-PARVALBUMIN IN THE GUINEA-PIG COCHLEA

Authors
SOTOPRIOR A CLUZEL M RENARD N RIPOLL C LAVIGNEREBILLARD M EYBALIN M HAMEL CP
Citation
A. Sotoprior et al., MOLECULAR-CLONING AND EXPRESSION OF ALPHA-PARVALBUMIN IN THE GUINEA-PIG COCHLEA, Molecular brain research, 34(2), 1995, pp. 337-342
Citations number
34
Categorie Soggetti
Neurosciences
Journal title
Molecular brain research → ACNP
ISSN journal
0169328X
Volume
34
Issue
2
Year of publication
1995
Pages
337 - 342
Database
ISI
SICI code
0169-328X(1995)34:2<337:MAEOAI>2.0.ZU;2-6
Abstract
We have cloned and sequenced an alpha parvalbumin cDNA from the guinea pig cochlea. The deduced amino acid sequence shows greater identity w ith the rabbit sequence (86.3%) than with other mammalian sequences (< 82%). Using in situ hybridization and immunohistochemistry, alpha par valbumin mRNA and protein were found in primary auditory neurons and i nner hair cells, in agreement with RT-PCR data showing alpha parvalbum in mRNA expression in the spiral ganglion and the organ of Corti.