A. Tsortos et al., THE DUAL ROLE OF FIBRINOGEN AS INHIBITOR AND NUCLEATOR OF CALCIUM-PHOSPHATE PHASES - THE IMPORTANCE OF STRUCTURE, Journal of colloid and interface science, 177(1), 1996, pp. 257-262
Constant composition and free drift methods have been used to investig
ate the abilities of human serum albumin (HSA) and fibrinogen to influ
ence calcium phosphate precipitation. Both molecules inhibit hydroxyap
atite (HAP) crystal growth when present in the solution phase. Fibrino
gen, when immobilized at hydrophobicized germanium or silica surfaces,
is able to nucleate calcium phosphate phases; at clean germanium or s
ilica surfaces, it appears to be inactive. The apparent configuration
of fibrinogen molecules at germanium or silica surfaces on which octad
ecyltrichlorosilane (OTS) was deposited probably exposes more negative
sites for participation in nucleation. (C) 1996 Academic Press, Inc.