Ma. Sanchezvalverde et al., METHOD OF RECORDING CEREBROSPINAL-FLUID PRESSURE AT 3 ANATOMIC PLACESIN CONSCIOUS AND UNRESTRAINED ADULT WISTAR RATS, Neuroscience research, 24(1), 1995, pp. 103-107
There does not appear to be a consensus on the normal values of cerebr
ospinal fluid pressure (Pcsf) in rats because of the different conditi
ons in which each experiment has been done. Anaesthesia, restraint or
recordings from different anatomical places and the use of simultaneou
s infusions could produce a great variability in the obtained results
and because of that the average Pcsf in rats has been reported to be b
etween 2 and about 18.5 cm H2O. The aim of this study is to provide ne
w information about the normal values of Pcsf in adult, conscious rats
by carrying out long-term measurements, for 3 consecutive days, from
different anatomical places. Thirty male Wistar rats divided in three
experimental groups of ten animals each were used. A catheter of silic
one was implanted into the lateral ventricles in group I, the cisterna
magna in group II, and the lumbar subarachnoid space in group III to
record the Pcsf values. This catheter was attached to a swivel system
to allow unrestrained and free behaviour in the rats. Mean values of n
ormal Pcsf values were 13.03 +/- 0.73 cm H2O from the lateral ventricl
es, 15.9 +/- 0.73 cm H2O from the cisterna magna, and 16.73 +/- 1.14 c
m H2O from the lumbar subarachnoid space.