Pl. Lopezdealba et al., SIMULTANEOUS DETERMINATION OF THE DYES YE LLOW-5 AND YELLOW-5 BY RATIO SPECTRA DERIVATIVE AND MEASUREMENT OF THE AREA OF THE RATIOS OF SPECTRA, Boletin de la Sociedad Chilena de Quimica, 40(4), 1995, pp. 393-401
In this work two spectrophotometric methods are described for the simu
ltaneous determination of yellow-5 (C.I. 19140) and yellow-6 (C.I. 159
85) in their mixtures: by first derivative of the ratio spectra and by
measuring the area of the ratio spectra. The proposed procedure does
not require any separation of the dyes prior to determination. Calibra
tion graphs for both dyes were linear up to 32 mu g . mL(-1). The reco
veries of Y5 and Y6 obtained in their mixtures (Y5:Y6 ratio were 0.5:7
and 7:0.5 (mim) respectively) were between 95 and 105% for both metho
ds. The proposed methods were applied for the determination of Y5 and
Y6 in different food products and satisfactory results were obtained.