BETA-ADRENERGIC MODULATION OF THE INWARDLY RECTIFYING POTASSIUM CHANNEL IN ISOLATED HUMAN VENTRICULAR MYOCYTES - ALTERATION IN CHANNEL RESPONSE TO BETA-ADRENERGIC STIMULATION IN FAILING HUMAN HEARTS

The beta-adrenergic modulation of the inwardly-rectifying K+ channel ( I-KI) was examined in isolated human ventricular myocytes using patch- clamp techniques, Isoproterenol (ISO) reversibly depolarized the resti ng membrane potential and prolonged the action potential duration. Und er the whole-cell Cl--free condition ISO applied via the bath solution reversibly inhibited macroscopic I-KI. The reversal potential of the ISO-sensitive current was shifted by similar to 60 mV per 10-fold chan ge in the external K+ concentration and was sensitive to Ba2+. The ISO -induced inhibition of l(KI) was mimicked by forskolin and dibutyryl c AMP, and was prevented by including a cAMP-dependent protein kinase (P KA) inhibitor (PKI) in the pipette solution, In single-channel recordi ngs from cell-attached patches, bath applied ISO could suppress I-KI c hannels by decreasing open state probability, Bath application of the purified catalytic subunit of PKA to inside-out patches also inhibited I-KI and the inhibition could be antagonized by alkaline phosphatase. When beta-adrenergic modulation of I-KI was compared between ventricu lar myocytes isolated from the failing and the nonfailing heart, chann el response to ISO and PKA was significantly reduced in myocytes from the failing heart, Although ISO inhibited I-KI in a concentration-depe ndent fashion in both groups, a half-maximal concentration was greater in failing (0.12 mu M) than in nonfailing hearts (0.023 mu M). These results suggest that I-KI in human ventricular myocytes can be inhibit ed by a PKA-mediated phosphorylation and the modulation is significant ly reduced in ventricular myocytes from the failing heart compared to the nonfailing heart.