K. Steinmeyer et al., CLONING AND FUNCTIONAL EXPRESSION OF RAT CLC-5, A CHLORIDE CHANNEL RELATED TO KIDNEY-DISEASE, The Journal of biological chemistry, 270(52), 1995, pp. 31172-31177
We have cloned a novel member of the CLC chloride channel family from
rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the
branch including CLC-3 and CLC-4, with which it shares similar to 80%
identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion
currents. They are strongly outwardly rectifying and have a conductiv
ity sequence of NO3- > Cl- > Br- > I- >> glutamate(-). Although CLC-5
has consensus sites for phosphorylation by protein kinase A, raising t
he intracellular cAMP concentration had no effect on these currents. C
urrents were also unchanged when rCLC-5 was coexpressed with rCLC-3 an
d rCLC-4, either singly or in combination, rCLC-5 is expressed predomi
nantly in kidney and also in brain, lung, and liver. Along the nephron
, rCLC-5 message is detectable in all tubule segments investigated, bu
t expression in the glomerulus and the S2 segment of the proximal tubu
le is low.