K. Saitoh et al., PRELIMINARY DATA ON RESTRICTION MAPPING AND DETECTION OF LENGTH VARIATION IN JAPANESE FLOUNDER MITOCHONDRIAL-DNA, Aquaculture, 136(1-2), 1995, pp. 109-116
We have mapped cleavage sites of ten restriction endonucleases in mito
chondrial DNA (mtDNA) of Japanese flounder, Paralichthys olivaceus. Hy
bridization with cloned or PCR-amplified fragments determined the map
position around structural genes of the mtDNA. Mapping experiments det
ected a high level of length variation including heteroplasmy near or
within the D-loop containing region. These results provide some basic
information for studies on genetic variation of the species important
in fisheries and aquaculture. Individual flounders used in this study
carry discrete types of mtDNA. The results implies mtDNA typing is use
ful for monitoring effectiveness or genetic impact of ongoing stocking
of the species into the natural habitat.