IMPROVEMENT OF A SOLID-PHASE EXTRACTION METHOD FOR DETERMINING BIOGENIC-AMINES IN WINES

Solid-phase extraction (SPE) was used simultaneously to clean up and c oncentrate samples prior to automatic derivatization to determine fift een biogenic amines in wine. In the first step, SPE was used to remove polyphenolic compounds which interfere with further extraction and ch romatographic analysis; in this study, treatments with polyvinylpyrrol idone, SAX and C-18 cartridges were tested and compared. In the second step, C-18 cartridges were used to concentrate the analytes after add ing sodium octanesulfonate, sodium decanesulfonate and sodium dodecane sulfonate as ion-pair reagents. Reversed-phase chromatography with flu orimetric detection was performed on the extracted amines after automa tic precolumn derivatization by treatment with o-phthalaldehyde, Bioge nic amines can be separated and detected after the solid-phase extract ion with an average sensitivity of the order of 20-90 mu g l(-1). Reco veries were determined by the standard addition technique and the over all method was successfully applied to the determination of the above- mentioned amines in red wines from the Tarragona region.