DEVELOPMENT OF DNA-IMMOBILIZED CHROMATOGRAPHIC STATIONARY PHASES FOR OPTICAL RESOLUTION AND DNA-AFFINITY COMPARISON OF METAL-COMPLEXES

Two chiral stationary phases which exploit DNA as a chiral discriminat or have been developed. A covalently-bound DNA stationary phase for HP LC applications was used to optically resolve [Ru(dipyrido[6,7-d:2',3' -f]-quinoxaline)(3)](2+) and [Ru(1,10-phenanthroline)(3)](2+) complex ions. This shows that the column retention times are influenced both b y pH and by the size of the aromatic ligands. DNA-immobilised on cellu lose paper proved effective for simultaneously comparing the relative retention of a number of metal complexes and R(F) data correlate well with the degree of aromatic area in the complexes available for interc alation into DNA.