Ga. Gordon, VOLTAGE PRECONDITIONING TECHNIQUE FOR OPTIMIZATION OF MIGRATION-TIME REPRODUCIBILITY IN CAPILLARY ELECTROPHORESIS, Journal of chromatography, 718(2), 1995, pp. 444-447
Citations number
9
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An additional tool for pre-conditioning a capillary electrophoretic sy
stem is described which involves the application of a short-term volta
ge (30 kV for 120 s) prior to analysis. Using this technique, migratio
n-time reproducibility was improved to less than or equal to 0.25% R.S
.D. (n = 10). This report comprises the first demonstration of the uti
lity and capillary-to-capillary reproducibility of such a technique. G
iven the buffering capacity of the electrolyte this is not due to pH s
tabilisation; other possible mechanisms are discussed.