DETERMINATION OF LIPASE ACTIVITY IN PORCINE PANCREAS AND CLINICAL ANALYSIS OF LIPASE IN HUMAN SERUM WITH SURFACE-ACOUSTIC-WAVE ENZYME SENSOR SYSTEM

A new kind of surface acoustic wave (SAW) enzyme sensor system has bee n applied to the assay of extracted lipase activity based on triacetin solution as substrate. A linear relationship between the frequency ch ange and the enzyme activity up to 500 U/L has been obtained, and the detection limit has been evaluated to 0.3 U/L. Kinetic parameters of t he extracted lipase as well as that of the standard enzyme have been e stimated. The effects of temperature, pH value and surfactants have be en investigated. A comparison between SAW sensor system and the conduc tometric method has been carried out. The technique has been applied t o the determination of lipase activity in serum samples.