K. Ge et al., DETERMINATION OF LIPASE ACTIVITY IN PORCINE PANCREAS AND CLINICAL ANALYSIS OF LIPASE IN HUMAN SERUM WITH SURFACE-ACOUSTIC-WAVE ENZYME SENSOR SYSTEM, Fresenius' journal of analytical chemistry, 354(1), 1996, pp. 118-121
A new kind of surface acoustic wave (SAW) enzyme sensor system has bee
n applied to the assay of extracted lipase activity based on triacetin
solution as substrate. A linear relationship between the frequency ch
ange and the enzyme activity up to 500 U/L has been obtained, and the
detection limit has been evaluated to 0.3 U/L. Kinetic parameters of t
he extracted lipase as well as that of the standard enzyme have been e
stimated. The effects of temperature, pH value and surfactants have be
en investigated. A comparison between SAW sensor system and the conduc
tometric method has been carried out. The technique has been applied t
o the determination of lipase activity in serum samples.