LOW CONCENTRATIONS OF OUABAIN INCREASE CYTOSOLIC-FREE CALCIUM-CONCENTRATION IN RAT VASCULAR SMOOTH-MUSCLE CELLS

Authors
ZHU ZM TEPEL M NEUSSER M ZIDEK W
Citation
Zm. Zhu et al., LOW CONCENTRATIONS OF OUABAIN INCREASE CYTOSOLIC-FREE CALCIUM-CONCENTRATION IN RAT VASCULAR SMOOTH-MUSCLE CELLS, Clinical science, 90(1), 1996, pp. 9-12
Citations number
25
Categorie Soggetti
Medicine, Research & Experimental
Journal title
Clinical science → ACNP
ISSN journal
01435221
Volume
90
Issue
1
Year of publication
1996
Pages
9 - 12
Database
ISI
SICI code
0143-5221(1996)90:1<9:LCOOIC>2.0.ZU;2-M
Abstract
1. Low ouabain concentrations in the nanomolar range significantly inc reased cytosolic free calcium concentration. 2. The ouabain-induced cy tosolic free calcium concentration increase was due to transplasmamemb rane calcium influx, which could be prevented in the absence of extrac ellular calcium or by addition of the calcium channel blocker nifedipi ne. 3. The amount of stored cellular Ca2+, as determined by the thapsi gargin-induced cytosolic free calcium concentration increase, was also enhanced by 1 nmol/l ouabain. 4. It is concluded that low ouabain con centrations affect intracellular cytosolic free calcium concentration homoeostasis.