INHERITANCE OF RECOMBINANT CARP BETA-ACTIN GH CDNA GENE IN TRANSGENICCARP

Individual carp (Cyprinus carpio) raised from zygotes that were microi njected with two different carp a-actin promoters fused to the chinook salmon GH cDNA gene. Twenty five individual fish, out of 200 males an d females, were shown to contain the constructs in a mosaic fashion at sexual maturity, and some of them were crossed with each other as wel l as with non-transgenic carp. The level of transgene transmittance fr om the transgenic parents varied between individuals. We observed 32%- 87% inheritance when transgenic parents were crossed and 0%-50% inheri tance when transgenic parents were mated with non-transgenics.