INDIRECT ENZYMATIC PHOSPHORYLATION - PREPARATION OF DIHYDROXYACETONE PHOSPHATE

Starting from natural phosphatidyl choline, the substitution of the ch oline polar head for an alcohol donor is catalysed by phospholipase D; subsequent hydrolysis of the new phospholipid obtained in the presenc e of phospholipase C allows the obtainment of the phosphate of the alc ohol donor; the overall process is the net transfer of the phosphate m oiety from a phospholipid to a primary alcohol and the procedure is ve rified in the preparation of dihydroxyacetone phosphate.