D1S80 VNTR LOCUS GENOTYPES IN POPULATION OF SOUTH POLAND - METAANALYSIS POINTER TO GENETIC DISEQUILIBRIUM OF HUMAN-POPULATIONS

A highly variable number of tandem repeats (VNTRs) in a human locus D1 S80 can prove to be useful for forensic science purposes. As with othe r genetic polymorphisms, a database of a local population allelic freq uencies is needed to ensure that no departure from genetic equilibrium exists. DNA from the locus D1S80 was amplified by polymerase chain re action (PCR) and analyzed by horizontal PAGE followed by silver staini ng. Samples from 133 unrelated inhabitants of Southern Poland were exa mined. The amplified fragment length polymorphism (AMP-FLP) analysis o f the D1S80 locus demonstrated 21 alleles and heterozygosity of 0.85%. Out of the 231 possible genotypes, 47 were observed. The results were compared to the published D1S80 population studies and a meta-analysi s of the genotype frequencies was performed. The G statistics revealed a deviation from genetic equilibrium in the Spanish population, Repli cated goodness of fit tests showed highly significant heterogeneity of genotype distribution between tested populations. Therefore, interpre tation of the casework on the basis of D1S80 locus typing may be biase d by interpopulation differences.