ENANTIOSPECIFIC ANALYSIS - APPLICATIONS IN BIOANALYSIS AND METABOLISM

Enantiospecific analysis has a significant role in modem drug developm ent from discovery-chemistry to the clinical evaluation of novel compo unds. Chromatographic techniques, involving the use of either chiral d erivatizing agents or chiral stationary phases, represent the most com monly used approaches to enantiospecific analysis. The advantages and limitations of these two techniques are examined using the analysis of the enantiomers of the 2-arylpropionic acids (tiaprofenic acid and ib uprofen) and the chiral N-oxides of N-ethyl-N-methylaniline and pargyl ine, as representative examples for each approach The potential of bio sensors in enantiospecific analysis is addressed and some preliminary results on the development of an enantioselective biosensor for the an alysis of (S)-warfarin are presented.