Ba. Heinz et al., SIMPLE IN-VITRO TRANSLATION ASSAY TO ANALYZE INHIBITORS OF RHINOVIRUSPROTEASES, Antimicrobial agents and chemotherapy, 40(1), 1996, pp. 267-270
We have developed a simple in vitro translation method to analyze comp
ounds that inhibit the rhinovirus 3C protease in peptide substrate ass
ays but demonstrate no antiviral activity. This complementary assay, w
hich provides both qualitative and quantitative results, detects the i
nhibition of the 3CD protease in the native polyprotein form.