P. Ekstrom et Lm. Ohlin, ONTOGENY OF GABA-IMMUNOREACTIVE NEURONS IN THE CENTRAL-NERVOUS-SYSTEMIN A TELEOST, GASTEROSTEUS-ACULEATUS L, Journal of chemical neuroanatomy, 9(4), 1995, pp. 271-288
The inhibitory neurotransmitter gamma-aminobutyric acid (GABA) is know
n to exert various neurotrophic actions in the developing nervous syst
em, but little is known about its distribution in the central nervous
system during early development. We have studied the development of GA
BA-immunoreactive (GABAir) neurons during embryogenesis of a teleost f
ish, the three-spined stickleback. As early as 51 h postfertilization
(PF; hatching occurs 144-168 h PF, and the first monoaminergic neurons
appear around 72 h PF) GABAir neurons appear in the ventral prosencep
halon caudal to the optic recess, in the ventral mesencephalon, and in
the spinal cord. Then, there is a gradual addition of GABAir cell gro
ups in the rostral prosencephalon and ventral rhombencephalon (66 h PF
), dorsal and caudal hypothalamus and pretectum (72 h PF), ventral hyp
othalamus (78 h PF), preoptic region, thalamus, and in the mesencephal
on and rhombencephalon (96 h PF). GABAir axons appear in the spinal co
rd already at 51 h PF, and then gradually appear in the various tracts
of the early axonal scaffold of pathfinding fibers, so that by 96 h P
F the entire axonal scaffold contains GABAir fibers. It appears likely
that GABAergic axons contribute a major population to the formation o
f the axonal scaffold. Moreover, in the prosencephalon GABAir neurons
are arranged in clusters that may reflect a neuromeric organization wi
th six prosencephalic neuromeres.