Hh. Iversen et al., MODULATION OF SMOOTH-MUSCLE ACTIVITY BY NITRIC-OXIDE IN THE HUMAN UPPER URINARY-TRACT, Urological research, 23(6), 1995, pp. 391-394
The aim of the study was to ascertain whether nitric oxide (NO) might
regulate motility in the human upper urinary tract. Smooth muscle acti
vity in the human renal pelvis and proximal ureter was studied in vitr
o in organ baths, and nitric oxide synthase (NOS) activity was studied
by measurement of citrulline formation. NO, glyceryl trinitrate (GTN)
and sodium nitroprusside (SNP) significantly reduced the frequency of
spontaneous rhythmic contractions in renal pelvis and proximal ureter
. Exogenously applied NO elicited relaxations in pre-contracted renal
pelvis. Calcium-dependent NOS activity was significant in the renal pe
lvis but undetectable in the ureter. Also, NOS activity was absent in
hydronephrotic renal pelvis. NO, SNP and GTN inhibited smooth muscle a
ctivity in the human upper urinary tract. NOS activity was obtained in
normal renal pelvis but not in hydronephrotic renal pelvis. Regulatio
n of urinary tract NO concentrations might offer a strategy for treatm
ent of renal colic and disturbances in upper urinary tract motility.