J. Leiro et al., THE HUMORAL IMMUNE-RESPONSE OF TURBOT TO RECENTLY ISOLATED PATHOGENICENTEROCOCCUS STRAINS - CROSS-REACTIVITY WITH OTHER GRAM-POSITIVE BACTERIA, Veterinary microbiology, 48(1-2), 1996, pp. 29-39
An Enterococcus sp. causing severe mortalities among farmed turbot (Sc
ophthalmus maximus) has recently been detected in northwest Spain. We
found that specific turbot serum antibodies raised against isolate RA-
99.1 of the new pathogen by intraperitoneal immunization, did not cros
s react in enzyme-linked immunosorbent assay (ELISA) with other entero
coccal or non-enterococcal Grampositive bacteria. In immunoblotting, a
ntibodies raised against strain RA-99.1 recognized the same components
in the homologous total soluble antigen preparation (TSA) as in TSA o
f two other isolates of the pathogen obtained from different farms. An
ti-RA-99.1 serum also recognized some components of the TSA of several
other Gram-positive bacteria. Immunogold labelling indicated that the
antigens which provoke the humoral immune response to this pathogen a
re located mainly on the bacterial surface.