THE HUMORAL IMMUNE-RESPONSE OF TURBOT TO RECENTLY ISOLATED PATHOGENICENTEROCOCCUS STRAINS - CROSS-REACTIVITY WITH OTHER GRAM-POSITIVE BACTERIA

Citation
J. Leiro et al., THE HUMORAL IMMUNE-RESPONSE OF TURBOT TO RECENTLY ISOLATED PATHOGENICENTEROCOCCUS STRAINS - CROSS-REACTIVITY WITH OTHER GRAM-POSITIVE BACTERIA, Veterinary microbiology, 48(1-2), 1996, pp. 29-39
Citations number
23
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
ISSN journal
03781135
Volume
48
Issue
1-2
Year of publication
1996
Pages
29 - 39
Database
ISI
SICI code
0378-1135(1996)48:1-2<29:THIOTT>2.0.ZU;2-Y
Abstract
An Enterococcus sp. causing severe mortalities among farmed turbot (Sc ophthalmus maximus) has recently been detected in northwest Spain. We found that specific turbot serum antibodies raised against isolate RA- 99.1 of the new pathogen by intraperitoneal immunization, did not cros s react in enzyme-linked immunosorbent assay (ELISA) with other entero coccal or non-enterococcal Grampositive bacteria. In immunoblotting, a ntibodies raised against strain RA-99.1 recognized the same components in the homologous total soluble antigen preparation (TSA) as in TSA o f two other isolates of the pathogen obtained from different farms. An ti-RA-99.1 serum also recognized some components of the TSA of several other Gram-positive bacteria. Immunogold labelling indicated that the antigens which provoke the humoral immune response to this pathogen a re located mainly on the bacterial surface.