Y. Wasteson et al., CHARACTERIZATION OF TETRACYCLINE AND ERYTHROMYCIN RESISTANCE IN ACTINOBACILLUS-PLEUROPNEUMONIAE, Veterinary microbiology, 48(1-2), 1996, pp. 41-50
Minimum inhibitory concentrations to tetracycline and erythromycin wer
e determined for nineteen isolates of Actinobacillus pleuropneumoniae
of Norwegian origin. The isolates were screened for rRNA methylase det
erminants (Erm genes) and for the tetracycline resistance Tet B determ
inant, using oligonucleotide probes, polymerase chain reaction and hyb
ridization. Ten isolates (53%) carried the Erm C determinant, two isol
ates (10%) carried the Erm A determinant, four isolates (21%) carried
both the Erm A and the Erm C determinants, and three isolates (16%) ca
rried none of the Erm determinants examined. Eight isolates (45%) carr
ied the Tet B determinant. Selected isolates were shown to transfer th
e Erm C and Erm A determinants at a frequency of 10-(7)-10(-9) per rec
ipient cell. This is the first description of A, pleuropneumoniae carr
ying either Erm A, Erm C or/and Tet B determinants.