HYDROLYSIS RATES OF OLIVE OIL BY LIPASE IN A MONODISPERSED O W EMULSION SYSTEM USING MEMBRANE EMULSIFICATION/

Hydrolysis of olive oil in isooctane by lipase from Candida cylindrace a in an aqueous solution was carried out in a polydispersed emulsion s ystem prepared by a homogenizer and in a monodispersed emulsion system prepared by the shirasu-porous-glass (SPG) membrane emulsification me thod. An emulsion system consisting of both SDS and PVA at pH 7.8 was best suited for the reaction. In the monodisperscd emulsion, the dropl et diameter was controlled by the pore diameter of the SPG membrane to give droplets with a narrow diameter distribution range. The rate of hydrolysis was affected by the concentrations of olive oil and lipase, the interfacial area and the emulsion droplets diameter. The kinetic data were interpreted by the interfacial reaction model, in which deso rption of the product from the interface was the rate determining step . The equilibrium constant of the adsorption of Lipase at the interfac e were very small compared with that obtained in the isothermal adsorp tion equilibrium of lipase at the interface without a surfactant. The equilibrium constants of the reaction between lipase adsorbed at the i nterface and olive oil in the organic phase were nearly of the same or der as those obtained in the Lewis cell and the VibroMixer. The desorp tion rate constants of the product were very large compared with that in the Lewis cell.