MALEATE CIS-TRANS ISOMERASE FROM ARTHROBACTER SP TPU-5446

Authors
KATO Y YAMAGISHI J ASANO Y
Citation
Y. Kato et al., MALEATE CIS-TRANS ISOMERASE FROM ARTHROBACTER SP TPU-5446, Journal of fermentation and bioengineering, 80(6), 1995, pp. 610-612
Citations number
10
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
80
Issue
6
Year of publication
1995
Pages
610 - 612
Database
ISI
SICI code
0922-338X(1995)80:6<610:MCIFAS>2.0.ZU;2-P
Abstract
gram-positive aerobic bacterium, Arthrobacter sp. strain TPU 5446 with a high maleate cis-trans isomerase (MAI) activity, was isolated from soil. Enzyme activity was induced when the strain was cultivated in th e presence of maleic acid and yeast extract as the carbon and nitrogen sources, respectively. From a crude extract of the cells, the enzyme was partially purified by ammonium sulfate fractionation and DEAE-Toyo pearl column chromatography. The enzyme acted specifically on maleic a cid and produced fumaric acid stoichiometrically.