P-31 NMR IN-VIVO STUDY OF RAT-BRAIN ENERGY-METABOLISM AFTER FRONTAL-CORTEX INJURY - A METHOD BASED ON CONVOLUTED CORRELATION-MATRICES OF THE SPECTRAL DATA

Disturbances of energy metabolism in rat brain after frontal lobectomy and post-traumatic effects of monosialic ganglioside GM(1) on brain e nergy metabolism were studied by P-31 NMR in vivo. Frontal lobectomg c aused the statistically significant reversible decrease of ATP and inc rease of ADP during the compensatory period, In a presence of CM, thes e post-operational effects were not observed. The mean value of normal ized correlation coefficients between NMR-measured phosphomonoesters, P-i, pH, phosphocreatine, gamma-ATP beta-ADP, alpha-ATP+ alpha-ADP + N AD/NADH + diphosphodiesters and beta-ATP for a given group of animals (Z-index) was used to estimate brain energetic status. It has been sho wn that the variation of the Z-index correlates with the dynamics of l ocomotor activity during the compensatory period. The Z-index increase d by more than 100% for the subgroup with an increased moving activity and only by about 20% for the subgroup with a decreased activity. Mor eover, the compositions of these subgroups were almost the same as tho se from the solution of the reverse task, i.e., the studied group was divided into two subgroups by the Z-index. The Z-index is proposed as a possible criterion of pathology detection in brain energetics.