CHARACTERIZATION OF THE PSEUDOMONAS-AERUGINOSA TRANSPOSABLE BACTERIOPHAGE-D3112-A AND BACTERIOPHAGE-D3112-B GENES

The left end DNA of Mu-like transposable bacteriophage D3112 was seque nced from bp 2521 to bp 5483. Two large open reading frames were ident ified: ORF A (bp 2539-4611) and ORF B (bp 4626-5378). ORF A can encode a 690 amino acid, 78 kDa protein which is 44.4% similar to Mu transpo sase and ORF B can encode a 250 amino acid, 27 kDa protein, which is 4 6.4% similar to, though 62 amino acids shorter than, the Mu B protein. The cloned D3112 A gene exhibited activity on a mini-D3112-containing plasmid in Pseudomonas aeruginosa.