SPLICE VARIANTS OF TYPE-VIII ADENYLYL-CYCLASE - DIFFERENCES IN GLYCOSYLATION AND REGULATION BY CA2+ CALMODULIN/

Three alternatively spliced type VIII adenylyl cyclase messages have b een identified by cDNA cloning and am plification from rat brain cDNA. Type VIII-A was previously referred to simply as type VIII (Cali, J. J., Zwaagstra, J. C., Mons, N., Cooper, D. M. F., and Krupinski, J. (1 994) J. Biol. Chem. 269, 12190-12195). The types VIII-B and -C cDNAs d iffer from that of type VIII-A by deletion of 90 and 198 base pair exo ns, respectively, which encode a 30-amino acid extracellular domain wi th two consensus sites for N-linked glycosylation and a 66-amino acid cytoplasmic domain. Stable expression of types VIII-A, -B, and -C cDNA s in human embryonal kidney 293 (HEK-293) cells leads to the appearanc e of novel proteins, which are recognized by type VIII-specific antibo dies and which co-migrate with immunoreactive species detected on immu noblots of rat brain membranes. Types VIII-A and -C are modified by N- linked glycosylation, while type VIII-B is insensitive to treatment wi th N-glycosidase F. An influx of extracellular Ca2+ stimulates cAMP ac cumulation in HEK-293 cells stably expressing type VIII-A, -B, or -C, but not in control cells. Adenylyl cyclase activity of each of the var iants is stimulated by Ca2+/calmodulin and the EC(50) for activation o f type VIII-C is one fourth of that for either type VIII-A or -B. Type VIII-C also has a distinct K-m for substrate, which is approximately 4-12-fold higher than that for types VIII-A or -B depending on whether Mn2+ or Mg2+ is the counterion for ATP. The differences in the struct ural and enzymatic properties of these three variants are discussed.