Supernumerary chromosomes have been identified in the chromosome compl
ement of Nephrops norvegicus L. by C-banding and restriction endonucle
ase-induced banding. These chromosomes showed peculiar features of typ
ical B chromosomes in terms of heterochromatin content, asynapsis and
distorted segregation. The presence of these chromosomes can, at least
partially, account for the numerical variability already observed in
this species; 2n and n values determined by counting these chromosomes
separately gave a less variable number of chromosomes in the compleme
nt. Q-banding was useful to characterize some large B chromosomes with
intercalary and subtelomeric bands as well as entirely positive short
arms in some small submetacentric chromosomes. Nucleolar organizer re
gions have been identified in four pairs of chromosomes by silver stai
ning.