CLONING STRATEGIES USING A 3RD IRRELEVANT ENZYME SITE (TIES) TO OVERCOME CERTAIN CLONING PROBLEMS

Authors
ZENG M HUANG BR LAPEYRE JN
Citation
M. Zeng et al., CLONING STRATEGIES USING A 3RD IRRELEVANT ENZYME SITE (TIES) TO OVERCOME CERTAIN CLONING PROBLEMS, BioTechniques, 20(1), 1996, pp. 68
Citations number
12
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
20
Issue
1
Year of publication
1996
Database
ISI
SICI code
0736-6205(1996)20:1<68:CSUA3I>2.0.ZU;2-L
Abstract
We describe a cloning strategy, third irrelevant enzyme site (TIES), b ased on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES s trategy has made it possible to obtain the desired recombinant where t he corresponding approach using conventional recombinant DNA methodolo gies and two-component ligations failed to give the desire product.