EFFECTS OF BENZYLPENICILLIN ON GLUCOSE-UTILIZATION, MACROMOLECULE SYNTHESIS AND CELL-WALL PROTEINS OF LISTERIA-MONOCYTOGENES

When growing cells were incubated in the presence of 200 ppm benzylpen icillin (BP) for 30 min and then harvested for metabolic studies, the initial rate of glucose utilization by resting cells was reduced by 19 .6% compared to control cells, whereas the rates of protein, DNA and R NA synthesis were reduced by 40.7%, 55.0% and 57.5% respectively. Grow th in the presence of 100 ppm BP for 2 h was found to result in a mark ed depletion of cell wall proteins.