M. Moller et R. Harling, RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) PROFILING OF PLASMODIOPHORA-BRASSICAE, Letters in applied microbiology, 22(1), 1996, pp. 70-75
A technique is described for the preparation of DNA suitable for use i
n RAPD analysis from pure, sterile resting spores of Plasmodiophora br
assicae. Using this technique, random 10-base pair primers were applie
d to P. brassicae DNA from three single spore isolates. The resulting
profiles were compared with the race classification based on inoculati
on of the European Clubroot Differential (ECD) series of Brassica host
s. Out of 40 primers tested, 23 gave amplification products, three gav
e isolate-specific profiles and one a profile which corresponded with
the ECD race classification of the isolates. RAPD profiling can provid
e a faster means of race classification in P. brassicae.