RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) PROFILING OF PLASMODIOPHORA-BRASSICAE

A technique is described for the preparation of DNA suitable for use i n RAPD analysis from pure, sterile resting spores of Plasmodiophora br assicae. Using this technique, random 10-base pair primers were applie d to P. brassicae DNA from three single spore isolates. The resulting profiles were compared with the race classification based on inoculati on of the European Clubroot Differential (ECD) series of Brassica host s. Out of 40 primers tested, 23 gave amplification products, three gav e isolate-specific profiles and one a profile which corresponded with the ECD race classification of the isolates. RAPD profiling can provid e a faster means of race classification in P. brassicae.