Aa. Hunaiti et al., DEVELOPMENTAL STUDIES ON DROSOPHILA-MELANOGASTER GLUTATHIONE-S-TRANSFERASE AND ITS INDUCTION BY OXADIAZOLONE, Insect biochemistry and molecular biology, 25(10), 1995, pp. 1115-1119
Glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene
was detected in various developmental stages of Drosophila melanogaste
r, The specific activity of the enzyme was 110, 35, 25 and 15nmol/min/
mg protein in crude extracts prepared from eggs, larvae, pupae and adu
lt stages respectively, The enzymes from larval, pupal and adult stage
s were purified and compared. Incorporation of the widely used herbici
de oxadiazolone at concentrations of 375 and 563 part/million into the
culture media caused 4- and 2.5-fold increase in the enzyme activity
in pupal and adult stages respectively.